Riboflavin is a coenzyme involved in the energy-producing mitochondrial electron-transport chain. Some evidence indicates that impaired mitochondria oxygen metabolism in the brain may play a role in the pathology of chronic severe headache. A reduction in energy production in mitochondria has been shown to occur in chronic severe headache patients. Riboflavin works to enhance mitochondrial energy production, which has been shown to promote reduced headache frequency and severity.